Enter an organism name (or organism group name such as enterobacteriaceae, rodents), taxonomy id or select from the suggestion list as you type. Help Entrez query (optional) Help …

Jul 21, 2025 · The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence …

Go to the Primer BLAST submission form. Enter the target sequence in FASTA format or an accession number of an NCBI nucleotide sequence in the PCR Template section of the form.

Primer-BLAST was developed at NCBI to help users make primers that are specific to intended PCR target.

Primer choice influences the estimated microbial composition. A set of different 16S rRNA gene sequencing primer pairs covering one, two, or three of the variable regions V1 to V9 is …

Try "re-search for specific primers" feature on Primer-BLAST result page. When only non-specific primers are found, the result page offers the option to re-search for specific primers.

Users can design new primer pairs by entering the DNA template alone or they can design one primer by entering the template plus the other pre-existing primer. Primer-BLAST can check …

Here, we describe a novel empirical-based method, miPrimer, which greatly reduces primer dimerization and increases primer specificity by factoring various intrinsic primer properties …

The peer-reviewed literature contains references to tens, if not hundreds of thousands of oligonucleotide primer sequences for use with the polymerase chain reaction (PCR) and …

Apr 25, 2025 · Large language models (LLMs) have emerged as powerful tools in many fields, including clinical pharmacology and translational medicine. This paper aims to provide a …