Feb 26, 2025 · In an attempt to set up a standardized protocol for NK cell isolation and expansion, we present a thoroughly tested and validated protocol that can produce highly pure, viable, …

Here, we provide a rapid and straightforward protocol for the isolation and stimulation of primary NK cells or iPSC-derived NK cell-like cells, and subsequent detection of secreted cytokines …

This protocol includes detailed instructions for preparing necessary reagents and materials and then carrying out each step.

Abstract NK92 cells are an interleukin-2 IL2dependent natural killer NK) cell line derived from peripheral blood mononuclear cells of a 50-year-old white male with acute non-Hodgkin …

rkflow for primary human NK cell isolation, xeno‐free expansion, harvest, and cryop ments. However, it is challenging to obtain primary human NK cells at clinically relevant scale. Here, …

Nov 21, 2019 · In this study, we try to develop a simple, safe and economical method for ex vivo expansion and purification of NK cells from CB without cell sorting and feeder cells/multiple …

NK cell culture is defined as the in vitro process of isolating and expanding natural killer (NK) cells using specific cytokine combinations and/or feeder cells to enhance their activation and …

This book provides a broad collection of methodologies for natural killer (NK) cell research, in cell specification and functional assays.

Nov 9, 2023 · Here, we provide a protocol for the rapid expansion of mouse T cells, natural killer (NK) cells, and bone-marrow-derived macrophages (BMDMs). We describe steps for …

Natural killer (NK) cells have been shown to have important functions in anti-tumor responses and therefore have been used as adoptive immunotherapy for cancer. Here, we review the current …